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Journal Article

Photodynamic opening of the blood–brain barrier using different photosensitizers in mice

Authors

Semyachkina-Glushkovskaya,  Oxana
External Organizations;

Borisova,  Ekaterina
External Organizations;

Mantareva,  Vanya
External Organizations;

Angelov,  Ivan
External Organizations;

Eneva,  Ivelina
External Organizations;

Terskov,  Andrey
External Organizations;

Mamedova,  Aysel
External Organizations;

Shirokov,  Alexander
External Organizations;

Khorovodov,  Alexander
External Organizations;

Klimova,  Maria
External Organizations;

Agranovich,  Ilana
External Organizations;

Blokhina,  Inna
External Organizations;

Lezhnev,  Nikita
External Organizations;

/persons/resource/Juergen.Kurths

Kurths,  Jürgen
Potsdam Institute for Climate Impact Research;

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Fulltext (public)

applsci-10-00033-v2.pdf
(Publisher version), 2MB

Supplementary Material (public)
There is no public supplementary material available
Citation

Semyachkina-Glushkovskaya, O., Borisova, E., Mantareva, V., Angelov, I., Eneva, I., Terskov, A., Mamedova, A., Shirokov, A., Khorovodov, A., Klimova, M., Agranovich, I., Blokhina, I., Lezhnev, N., Kurths, J. (2020): Photodynamic opening of the blood–brain barrier using different photosensitizers in mice. - Applied Sciences, 10, 1, 33.
https://doi.org/10.3390/app10010033


Cite as: https://publications.pik-potsdam.de/pubman/item/item_24208
Abstract
In a series of previous studies, we demonstrated that the photodynamic therapy (PDT), as a widely used tool for treatment of glioblastoma multiforme (GBM), also site-specifically opens the blood–brain barrier (BBB) in PDT-dose and age-related manner via reversible disorganization of the tight junction machinery. To develop the effective protocol of PDT-opening of the BBB, here we answer the question of what kind of photosensitizer (PS) is the most effective for the BBB opening. We studied the PDT-opening of the BBB in healthy mice using commercial photosensitizers (PSs) such as 5-aminolevulenic acid (5-ALA), aluminum phthalocyanine disulfonate (AlPcS), zinc phthalocyanine (ZnPc) and new synthetized PSs such as galactose functionalized ZnPc (GalZnPc). The spectrofluorimetric assay of Evans Blue albumin complex (EBAC) leakage and 3-D confocal imaging of FITC-dextran 70 kDa (FITCD) extravasation clearly shows a revisable and dose depended PDT-opening of the BBB to EBAC and FITCD associated with a decrease in presence of tight junction (TJ) in the vascular endothelium. The PDT effects on the BBB permeability, TJ expression and the fluorescent signal from the brain tissues are more pronounced in PDT-GalZnPc vs. PDT-5-ALA/AlPcS/ZnPc. These pre-clinical data are the first important informative platform for an optimization of the PDT protocol in the light of new knowledge about PDT-opening of the BBB for drug brain delivery and for the therapy of brain diseases. View Full-Text